ASBC
METHODS OF ANALYSIS

Table of Contents

Barley Methods

Malt Methods

Adjunct Materials Methods

Brewers' Grains Methods

Hops Methods

Wort Methods

Beer Methods

Flavored Alcohol Beverages

Microbiology Methods

Filter Aids Methods

Packages and Packaging Materials Methods

By-Products Methods

Sensory Analysis Methods

Statistical Analysis Methods

Extract Tables

Identification Guides

Barley Methods

Glossary [Released 2007]
VIEW GLOSSARY

Barley 1. Sampling and Grading [Released 1958, Revised 2003]
VIEW SUMMARY | VIEW METHOD

Barley 2. Physical Tests  
VIEW SUMMARY | VIEW METHOD
A. Variety [Released 1977, Revised 2003]
B. Test Weight per Bushel (Bushelweight) [Released 1958, Revised 2003]
C. Assortment [Released 1958, Revised 2003]
D. 1,000-Kernel Weight  [Released 1958, Revised 2003]
E. Texture of Endosperm (Mellowness) [Released 1958, Revised 2003]
F. Skinned and/or Broken Kernels [Released 1958, Revised 2003]
G. Weathering and Kernel Damage [Released 1958, Revised 2003]
H. Injury by Sprout [Released 1958, Revised 2003]

Barley 3. Germination
VIEW SUMMARY | VIEW METHOD
A. Germinative Energy [Released 1958]
B. Germinative Capacity -- Hydrogen Peroxide Method (International Method) [Released 1978]
C. Germinative Energy, Germinative Capacity, and Water Sensitivity -- Simultaneous Determination [Released 1997]

Barley 4. Preparation of Sample for Chemical Analysis [Released 1958]
VIEW SUMMARY | VIEW METHOD

Barley 5. Moisture
VIEW SUMMARY | VIEW METHOD
A. Air-Oven Method  [Released 1958]
B. Alternate Air-Oven Method  [Released 1979]
C. Moisture in Whole Grain by Near-Infrared  [Released 1998]

Barley 6. Extract  [Released 1958]
VIEW SUMMARY | VIEW METHOD

Barley 7. Protein [Release date 2007]
VIEW SUMMARY | VIEW METHOD
A. Protein (N x 6.25) by Kjeldahl (International Method) [Released 1958]
B. Protein by Near-Infrared [Release date 1980]
C. Protein (N x 6.25) by Combustion [Released 1992]
D. Protein in Whole Grain by Near-Infrared [Released 1998]

Barley 8. Potential Diastatic Power [Released 1959]
VIEW SUMMARY | VIEW METHOD

Barley 9. Kernel Brightness (Instrumental) [Released 1979]
VIEW SUMMARY | VIEW METHOD

Barley 10. Barley Pregermination by Fluorescein Dibutyrate (International Method) [Released 1989]
VIEW SUMMARY | VIEW METHOD

Barley 11. Deoxynivalenol by Gas Chromatography [Released 1996]
VIEW SUMMARY | VIEW METHOD

Barley 12. Sprout Damage 
VIEW SUMMARY | VIEW METHOD
A. By Falling Number  [Released 2004]
B. By Stirring Number [Released 2004]

Malt Methods

Glossary [Released 2011]
VIEW GLOSSARY

Malt 1. Sampling  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Malt 2. Physical Tests  
VIEW SUMMARY | VIEW METHOD
A. Test Weight per Bushel (Bushelweight) [Released 2011]
B. Assortment  [Released 2011]
C. 1,000-Kernel Weight  [Released 2011]
D. Foreign Seeds and Skinned or Broken Kernels  [Released 2011]
E. Mealiness  [Release date 2011]
F. Length of Acrospire (Growth Count)  [Released 2011]

Malt 3. Moisture  [Released 1958]
VIEW SUMMARY | VIEW METHOD

Malt 4. Extract  [Released 1958, revised 2011]
VIEW SUMMARY | VIEW METHOD

Malt 6. Diastatic Power  
VIEW SUMMARY | VIEW METHOD
A. Diastatic Power  [Released 2011]
B. Diastatic Power (Rapid Method)  [Released 2011]
C. Automated Flow Analysis  [Released 2011]

Malt 7. Alpha-Amylase  
VIEW SUMMARY | VIEW METHOD
A. Fixed Color and Variable Time Method (International Method)  [Released 2011]
B. Fixed Time and Variable Color Method  [Released 2011]
C. Automated Flow Analysis Method Using Iodine Reagent [Released 2011]
D. Automated Flow Analysis Method Using Potassium Ferricyanide Reagent  [Released 2013]

Malt 8. Protein (N X 6.25)  
VIEW SUMMARY | VIEW METHOD
A. By Kjeldahl (International Method)  [Released 2011]
B. By Combustion  [Release 2011]

Malt 9. High-Dried, Caramel, and Black Malts (Caramel Extract is International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Malt 10. N-Nitrosamines  
VIEW SUMMARY | VIEW METHOD
A. Vacuum Distillation Method  [Released 2011]
B. Celite Column Extraction  [Released 2011]
C. Hot Aqueous Extraction  [Released 2011]

Malt 11. Sulfur Dioxide  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Malt 12. Malt Modification by Friability (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Malt 13. Deoxynivalenol by Gas Chromatography  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Malt 14. Dimethyl Sulfide Precursor by Headspace Gas Chromatography  
[Released 2011]
VIEW SUMMARY | VIEW METHOD

Malt 15. Grist  
VIEW SUMMARY | VIEW METHOD
A. By Standard Sieve  [Released 2011]
B. By Manual Sieve  [Released 2011]

Malt 16. Lipoxygenase Enzymatic Activity In Malted Barley By Absorbance Method  [Released 2017]
VIEW SUMMARY | VIEW METHOD

Adjunct Materials Methods

Glossary [Released 2007]
VIEW GLOSSARY

Introduction 
VIEW INTRODUCTION

Cereals

Cereals 1. Sampling  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Cereals 2. Physical Characteristics  
VIEW SUMMARY | VIEW METHOD
Removal of Accidental Foreign Particles  [Release date 1958]
Color  [Release date 1958]
Odor  [Release date 1958]
Husks, Germs, and Foreign Seeds  [Release date 1958]
Mold  [Release date 1958]
Insect Infestation  [Release date 1958]
Assortment of Corn Grits  [Release date 1963]

Cereals 3. Moisture (International Method)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Cereals 4. Oil (Fatty Substances)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Cereals 5. Extract  
VIEW SUMMARY | VIEW METHOD
A. Malt Conversion Method (International Method)  [Release date 1958]
B. Enzyme Conversion Method for Corn Grits (International Method)  [Release date 1963]

Cereals 6. Protein (N X 6.25)  
VIEW SUMMARY | VIEW METHOD
A. By Kjeldahl  [Release date 1958]
B. By Combustion  [Release date 1992]

Cereals 7. Ash  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups

Sugars and Syrups 1. Sampling  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 2. Physical Characteristics (Color, Odor, Taste)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 3. Clarity of "10% Solution"*  [Release date 1958]

Sugars and Syrups 4. Color of "10% Solution" (International Method)*  [Release date 1958]

Sugars and Syrups 5. Extract (International Method)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 6. Moisture  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 7. Fermentable Extract  
VIEW SUMMARY | VIEW METHOD
A. By Regular Fermentation  [Release date 1958]
B. By Rapid Fermentation 1958 [Release date 1958]

Sugars and Syrups 8. Iodine Reaction  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 9. Acidity  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 10. pH (Hydrogen Ion Concentration)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 11. Protein (N X 6.25)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 12. Ash  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 13. Diastatic Power (Diastatic Malt Syrups Only)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 14. Total Reducing Sugars  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 15. Sucrose  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 16. Dextrose in Presence of Other Reducing Sugars  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 17. Fermentable Saccharides by Chromatography 
VIEW SUMMARY | VIEW METHOD
A. Gas Chromatography Method  [Release date 1977]
B. High-Performance Liquid Chromatography Method 1977 [Release date 1977]

Sugars and Syrups 18. Fermentable Carbohydrates by Cation Exchange HPLC  [Release date 1997]
VIEW SUMMARY | VIEW METHOD

Brewers' Grains Methods

Glossary [Release date 2007]
VIEW GLOSSARY

Brewers' Grains 1. Sampling  
VIEW SUMMARY | VIEW METHOD
A. Wet Brewers' Grains  [Release date 1958]
B. Dry Brewers' Grains  [Release date 1958

Brewers' Grains 2. Preparation of Sample  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Brewers' Grains 3. Moisture  
VIEW SUMMARY | VIEW METHOD
A. On Sample in Wet Condition  [Release date 1958]
B. On Sample After Preliminary Drying  [Release date 1958]
C. On Dry Brewer' Grains  [Release date 1958]
D. On Sample in Wet Condition—Rapid Method  [Release date 1995

Brewers' Grains 4. Available Extract  
VIEW SUMMARY | VIEW METHOD
A. Wet Brewers' Grains  [Release date 1958]
B. Dry Brewers' Grains  [Release date 1958]

Brewers' Grains 5. Soluble Extract  
VIEW SUMMARY | VIEW METHOD
A. Wet Brewers' Grains  [Release date 1958]
B. Dry Brewers' Grains  [Release date 1958]

Brewers' Grains 6. Feed Analysis  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Brewers' Grains 7. Protein (N x 6.25) by Combustion  [Release date 1992]
VIEW SUMMARY | VIEW METHOD

Hops Methods

Glossary [Release date 2007]
VIEW GLOSSARY

Hops 1. Sampling  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 2. Physical Examination  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 3. Aphids in Hops  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 4. Moisture  
VIEW SUMMARY | VIEW METHOD
A. Moisture by Distillation  [Release date 2010]
B. Moisture by Vacuum Drying  [Release date 2010]
C. Moisture by Routine Air Oven Method  [Release date 2010]

Hops 5. Resins  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 6. α-and β-Acids in Hops and Hop Pellets  
VIEW SUMMARY | VIEW METHOD
A. α-and β-Acids by Spectrophotometry  [Release date 2010]
B. Conductometric Value  [Release date 2010]

Hops 7. α-Acids in Hops and Hop Pellets by Ion-Exchange Chromatography*  
A. α-Acids Using Sephadex Ion-Exchange Resin*  [Release date 1976]
B. α-Acids Using Dowex Ion-Exchange Resin*  [Release date 1976]

Hops 8. Non-Isomerized Hop Extracts  
VIEW SUMMARY | VIEW METHOD
A. Isooctane Spectrophotometric Method*  [Release date 1970]
B. Isopropyl Ether Spectrophotometric and Conductometric Methods  [Release date 2010]

Hops 9. Isomerized Hop Extracts  
VIEW SUMMARY | VIEW METHOD
A. Iso-α-Acids and rho-Iso-α-Acids by Dowex Ion-Exchange Chromatography* 1974  [Release date 1958]
B. Iso-α-Acids by Sephadex Stepwise Ion-Exchange Chromatography (International Method)* 1979  [Release date 1958]
C. Iso-α-Acids by HPLC  [Release date 2010]
D. Iso-α-Acids by HPLC  [Release date 2010]

Hops 10. Hop Bitter Acids in Non-Isomerized Extracts by Stepwise Ion-Exchange Chromatography*  
A. α-Acids by Dowex Ion-Exchange Chromatography*  [Release date 1977]
B. α-Acids by Sephadex Ion-Exchange Chromatography*  [Release date 1977]
C. β-Acids by Sephadex Ion-Exchange Chromatography*  [Release date 1978]

Hops 11. Gradient Elution of Hop Constituents Using Sephadex Ion-Exchange Chromatography*  [Release date 1978]

Hops 12. Hop Storage Index  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 13. Total Essential Oils in Hops and Hop Pellets by Steam Distillation  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 14. α-Acids and β-Acids in Hops and Hop Extracts by HPLC (International Method)  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 15. Iso-α-Acids in Isomerized Hop Pellets by HPLC 1993  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 16. Iso-α, α-, and β-Acids in Hop Extracts and Isomerized Hop Extracts by HPLC  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 17. Hop Essential Oils by Capillary Gas Chromatography-Flame Ionization Detection  [Release date 2004]
VIEW SUMMARY | VIEW METHOD

Hops 18. Determination Of Tetrahydroiso-α-Acids In Hop Products By Spectrophotometry [Release date 2017]
VIEW SUMMARY | VIEW METHOD

Wort Methods

Glossary [Release date 2011]
VIEW GLOSSARY

Wort 1. Sampling  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 2. Specific Gravity  
VIEW SUMMARY | VIEW METHOD
A. By Pycnometer*  [Release date 1958]
B. By Digital Density Meter  [Release date 2011]

Wort 3. Extract  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 4. Apparent Extract by Hydrometer  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 5. Yeast Fermentable Extract  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 6. Iodine Reaction*  [Release date 1958]

Wort 7. Total Acidity  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 8. pH (Hydrogen Ion Concentration)  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 9. Wort Color and Sample Preparation  
VIEW SUMMARY | VIEW METHOD
A. Celite  [Release date 2011]
B. Filter Paper or Disks  [Release date 2011]

Wort 10. Protein  
VIEW SUMMARY | VIEW METHOD
A. By Kjeldahl  [Release date 2011]
B. By Combustion  [Release date 2011]

Wort 11. Reducing Sugars (Copper Reducing Substances)  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 12. Free Amino Nitrogen (International Method)  [Release date 2011]
VIEW SUMMARY | VIEW METHOD
A. Ninhydrin Method  [Release date 2011]
B. Segmented Flow Analysis [Release date 2011]

Wort 13. Viscosity  
VIEW SUMMARY | VIEW METHOD
A. Viscometer Method  [Release date 2010]
B. Rolling Ball Method  [Release date 2013]

Wort 14. Fermentable Saccharides by Chromatography  
VIEW SUMMARY | VIEW METHOD
A. Gas Chromatography Method  [Release date 2011]
B. High-Performance Liquid Chromatography Method (International Method)  [Release date 2011]

Wort 15. Magnesium by Atomic Absorption Spectrophotometry  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 16. Zinc by Atomic Absorption Spectrophotometry (International Method)  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 17. Protein in Unhopped Wort by Spectrophotometry  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 18. β-Glucan in Congress Wort [Release date 2011]
VIEW SUMMARY | VIEW METHOD
A. Fluorescence [Release date 2011]
B. Segmented Flow Analysis [Release date 2011]

Wort 19. Fermentable Carbohydrates by Cation Exchange HPLC  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 20. Elemental Analysis by Inductively Coupled Plasma-Atomic Emission Spectroscopy  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 21. Thiobarbituric Acid Index 2009  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Wort 22. Wort and Beer Fermentable and Total Carbohydrates by HPLC [Release date 2012]
VIEW SUMMARY | VIEW METHOD

Wort 23. Wort Bitterness [Release date 2012]
VIEW SUMMARY | VIEW METHOD
A. Bitterness Units by Spectrophotometry [Release date 2011]
B. Bitterness Units by Segmented Flow Analysis [Release date 2011]
C. Iso-α-Acids in Wort by HPLC [Release date 2011]

Wort 24. International Bitterness Units in Wort by Spectrophotometer 
VIEW SUMMARY

Wort 25. Rapid Malt Color [Release date 2016]
VIEW SUMMARY | VIEW METHOD

Beer Methods

Recommended Beer Degassing Methods and Alternatives Matrix
VIEW SUMMARY | VIEW MATRIX

Recommended Beer Degassing Methods and Alternatives - Comparison Chart
VIEW SUMMARY | VIEW CHART


Glossary [Release date 2006]
VIEW GLOSSARY

Beer 1. Sampling  
VIEW SUMMARY | VIEW METHOD
A. Preparation of Sample for Chemical and Physical Analysis  [Release date 1958, reviewed 2018]
B. Aseptic Sampling from Tanks and Lines  [Release date 1966, reviewed 2018]
C. Aseptic Sampling from Finished Packages  [Release date 1971, reviewed 2018]
D. Decarbonation by a Rotary Shaker  [Release date 1998, reviewed 2018]

Beer 2. Specific Gravity 
Reviewed 2014
VIEW SUMMARY | VIEW METHOD
A. By Pycnometer*  [Released 1958; Revised 1975; Archived 1992]
B. By Digital Density Meter  [Released 1978; Revised 1981, reviewed and revised 2014]

Beer 3. Apparent Extract  [Release date 1958, revised 1975, reviewed and revised 2014]
VIEW SUMMARY | VIEW METHOD

Beer 4. Alcohol  
VIEW SUMMARY | VIEW METHOD
A. Beer and Distillate Measured Volumetrically  [Release date 1958, reviewed 2018]
B. Beer and Distillate Measured Gravimetrically  [Release date 1958, reviewed 2018]
C. Alcohol Determined Refractometrically  [Release date 1965, reviewed 2018]
D. Ethanol Determined by Gas Chromatography  [Release date 1979, reviewed 2018]
E. Instrumental Method for Alcohol and Original Gravity Content  [Release date 1989, reviewed 2018]
F. Enzymatic Method for Low Alcohol Concentrations (Intl. Method)  [Release date 1991, reviewed 2018]
G. Near-Infrared and Original Extract Content  [Release date 2004, reviewed 2018]

Beer 5. Real Extract  
VIEW SUMMARY | VIEW METHOD
A. Beer Measured Volumetrically  [Release date 1958, Reviewed 2018]
B. Beer Measured Gravimetrically  [Release date 1958, Reviewed 2018]
C. Real Extract Determined Refractometrically  [Release date 1982, Reviewed 2018]

Beer 6. Calculated Values  
VIEW SUMMARY | VIEW METHOD
A. Extract of Original Wort  [Release date 1958, Reviewed 2018]
B. Real Degree of Fermentation  [Release date 1958, Reviewed 2018]
C. Apparent Degree of Fermentation  [Release date 1958, Reviewed 2018]
D. Carbohydrate Content of Beer  [Release date 1978, Reviewed 2018]

Beer 7. Iodine Reaction* 
A. For Pale Beers*  [Release date 1958]
B. For Dark Beer but Also Applicable to Light Beers*  [Release date 1958]

Beer 8. Total Acidity  
VIEW SUMMARY | VIEW METHOD
A. By Potentiometric Titration  [Release date 1958, reviewed 2016]
B. By Titration of Diluted Beer with Phenolphthalein as Indicator  [Release date 1958, reviewed 2016]

Beer 9. pH (Hydrogen Ion Concentration)  [Release date 1958, reviewed 2018]
VIEW SUMMARY | VIEW METHOD

Beer 10. Color  
VIEW SUMMARY | VIEW METHOD
A. Spectrophotometric Color Method  [Release date 1958, revised 1975, reviewed 2015]
B. Photometric Method (Instruments other than precision spectrophotometers)  [Release date 1958, revised 1975, reviewed 2015]
C. Tristimulus Analysis (Colorimetric or Spectrophotometric)  [Release date 2002, reviewed 2015]

Beer 11. Protein   
VIEW SUMMARY | VIEW METHOD
A. By Kjeldahl  [Release date 1958, revised 1975, 1990, reviewed 2015]
B. By Combustion  [Release date 1993, reviewed 2015]
C. By Spectrophotometer  [Release date 2005, revised 2013, reviewed 2015]

Beer 12. Reducing Sugars (Copper Reducing Substances)  
VIEW SUMMARY | VIEW METHOD
A. Munson and Walker Method  [Release date 1958]
B. Lane-Eynon Volumetric Method  [Release date 1958]

Beer 13. Dissolved Carbon Dioxide  
VIEW SUMMARY | VIEW METHOD
A. Pressure Method for Beer in Tanks  [Release date 1958]
B. Pressure Method for Beer in Bottles and Cans  [Release date 1958]
C. Manometric/Volumetric Method  [Release date 1993]
D. Volume Expansion Method  [Release date 2009]

Beer 14. Ash 1958 [Release date 1958, revised 1975, reviewed 2015]
VIEW SUMMARY | VIEW METHOD

Beer 15. Total Phosphorus*  [Release date 1958]

Beer 16. End Fermentation (Yeast Fermentable Extract)  [Released 1958, revised 1975, reviewed 2015]
VIEW SUMMARY | VIEW METHOD

Beer 17. Dextrins*  [Released 1958]

Beer 18. Iron 
VIEW SUMMARY | VIEW METHOD
A. Analysis by Colorimetry (International)  [Released 1958; Revised 1975, reviewed 2015]
B. Analysis by Atomic Absorption Spectrophotometry (International)  [Released 1974; Revised 1975, reviewed 2015]
C. Analysis by Ferrozine  [Released 1992; reviewed and revised 2015]


Beer 19. Copper*  
VIEW SUMMARY | VIEW METHOD
A. By ZDBT Method (International Method)  [Release date 1958]*
B. Cuprethol Method 1969 [Release date 1978]*
C. Analysis by Atomic Absorption Spectrophotometry (International Method)  [Release date 1974]*

Beer 20. Calcium 
VIEW SUMMARY | VIEW METHOD
A. Chrome Black T Indicator Method  [Release date 1972, revised 1975, reviewed and revised 2015]
B. Calcein Indicator Method (Direct Titration)  [Release date 1972, reviewed and revised 2015]
C. Analysis by Atomic Absorption Spectrophotometry (International Method)  [Released 1972, revised 1975, reviewed and revised 2015]

Beer 21. Total Sulfur Dioxide  
VIEW SUMMARY | VIEW METHOD
A. p-Rosaniline Method  [Release date 1975]
B. Segmented Flow Analyzer Method (International Method) [Release date 2009]

Beer 22. Foam Collapse Rate 
VIEW SUMMARY | VIEW METHOD
A. Sigma Value Method (Modified Carlsberg Method)  [Release date 1962]
B. Foam Flashing Method  [Release date 1962]

Beer 23. Beer Bitterness 
VIEW SUMMARY | VIEW METHOD
A. Bitterness Units (International Method)  [Release date 1968, revised 1975, Revised 2018]
B. Iso-α-Acids by Solvent Extraction* [Release date 1968, Revised 2011, Archived 2018]
C. Iso-α-Acids by Solid-Phase Extraction and HPLC  [Release date 1993, Revised 2018]
D. Bitterness by Automated Flow Analysis  [Release date 1995, Revised 2018]
E. Iso-α-Acids in Beer by HPLC  [Release date 2011, Revised 2018]
F. Iso-α-Acids by Solvent Extraction [Release date 2018]

Beer 24. n-Heptyl p-Hydroxybenzoate* 
A. By Gas-Liquid Chromatography*  [Release date 1972]
B. By Ultraviolet Spectrophotometry (Nonspecific Method)*  [Release date 1972]

Beer 25. Diacetyl 
VIEW SUMMARY | VIEW METHOD
A. Macro Dimethyl Glyoxime Method*  [Release date 1964]
B. Broad Spectrum Method for VDK  [Release date 1964]
C. Micro Dimethyl Glyoxime Method  [Release date 1964]
D. Ultraviolet Spectrophotometer Method*  [Release date 1964]
E. Gas Chromatographic Method*  [Release date 1964]
F. Gas Chromatographic Method B  

Beer 26. Formazin Turbidity Standards  [Release date 1958, revised 1975, reviewed and revised 2015]
VIEW SUMMARY | VIEW METHOD

Beer 27. Physical Stability 
VIEW SUMMARY | VIEW METHOD
I. Total Haze after Chilling 
   A. Visual Method  [Release date 1962]
   B. Nephelometric Method  [Release date 1962]
II. Accelerated Chill-Haze Test 
   A. Elevated Temperature Storage Prior to Chilling  [Release date 1965]

Beer 28. Qualitative Test for Detection of Chillproofing Enzymes in Beer*  [Release date 1967]

Beer 29. Lower Boiling Volatiles in Beer or Ale  [Release date 1973, Revised 2018]
VIEW SUMMARY | VIEW METHOD

Beer 30. Testing for Taste Difference Between Two Beers  [Release date 1969]
VIEW SUMMARY | VIEW METHOD

Beer 31. Free Amino Nitrogen (International Method)  [Release date 1975]
VIEW SUMMARY | VIEW METHOD

Beer 32. Viscosity (International Method)  [Release date 1975]
VIEW SUMMARY | VIEW METHOD

Beer 33. Caloric Content (Calculated)  [Release date 1970]
VIEW SUMMARY | VIEW METHOD

Beer 34. Dissolved Oxygen  [Release date 1976]
VIEW SUMMARY | VIEW METHOD

Beer 35. Total Polyphenols (International Method)  [Released 1978, reviewed and revised 2015]
VIEW SUMMARY | VIEW METHOD

Beer 36. Sodium by Atomic Absorption Spectrophotometry (International Method) 
[Release date 1978, revised 1981, reviewed and revised 2015]
VIEW SUMMARY | VIEW METHOD

Beer 37. Potassium by Atomic Absorption Spectrophotometry (International Method)  [Released 1978, revised 1981, reviewed and revised 2015]
VIEW SUMMARY | VIEW METHOD

Beer 38. Magnesium and Calcium 
VIEW SUMMARY | VIEW METHOD
A. Magnesium by Atomic Absorption Spectrophotometry (International Method)  [Release date 1982]
B. Calcium and Magnesium by Sequential Titration  [Release date 1985]

Beer 39. Chloride 
VIEW SUMMARY | VIEW METHOD
A. Potentiometer Titration*  [Released 1980, archived 1992]
B. Conductometric Titration (International Method)  [Released 1980, reviewed 2015]
C. Mercurimetric Titration  [Released 1980, reviewed and revised 2015]

Beer 40. N-Nitrosamines 
VIEW SUMMARY | VIEW METHOD
A. Distillation  [Release date 1981, Reviewed 2018]
B. Celite Adsorption  [Release date 1965, Reviewed 2018]

Beer 41. Total Carbohydrate 
VIEW SUMMARY | VIEW METHOD
A. By Spectrophotometry  [Released 1983, Revised 2018]
B. By HPLC  [Released 1990, Revised 2018]

Beer 42. Aluminum by Graphite Furnace Atomic Absorption Spectrophotometry  [Released 1992, reviewed and revised 2017]
VIEW SUMMARY | VIEW METHOD

Beer 43. Anions (Chloride, Phosphate, and Sulfate) by Ion Chromatography (International Method)  [Released 1994, Reviewed 2018]
VIEW SUMMARY | VIEW METHOD

Beer 44. Dimethyl Sulfide by Chemiluminescence Detection  
[Released 1994, reviewed and revised 2015]
VIEW SUMMARY | VIEW METHOD

Beer 45. Elemental Analysis by Inductively Coupled Plasma-Atomic Emission Spectroscopy  [Released 2007]
VIEW SUMMARY | VIEW METHOD

Beer 46. Measurement of Oxidative Resistance in Beer by Electron Paramagnetic Resonance  [Released 2009, reviewed and revised 2016]
VIEW SUMMARY | VIEW METHOD

Beer 47. Iso-Alpha-Acids In Beer and Wort by HPLC
VIEW SUMMARY

Beer 48. Headspace Gas Chromatography–Flame Ionization Detection Analysis of Beer Volatiles [Released 2013]
VIEW SUMMARY | VIEW METHOD

Beer 49. Determination of Gluten Using the R5 Competitive ELISA Method [Released 2013]
VIEW SUMMARY | VIEW METHOD

Beer 50. Foam Stability by Nibem [Released 2016]
VIEW SUMMARY | VIEW METHOD

Beer Inclusions: Common Causes of Elevated Turbidity  [Released 2008]
VIEW SUMMARY | VIEW METHOD

Flavored Alcohol Beverages

Glossary  [Release date 2007]
VIEW GLOSSARY

Flavored Alcohol Beverages 1. Alcohol and Original Extract Content in Flavored Alcohol Beverages by Near-Infrared [Released 2007]
VIEW SUMMARY | VIEW METHOD

Microbiology Methods

Glossary  [Released 2007]
VIEW GLOSSARY

Introduction  [Released 2007]
VIEW INTRODUCTION

Yeast

Yeast 1. Sampling  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 2. Yeast Examination 
VIEW SUMMARY | VIEW METHOD
A. Cell Morphology  [Released 2011]
B. Presence of Bacteria  [Released 2011]

Yeast 3. Yeast Stains 
VIEW SUMMARY | VIEW METHOD
A. Dead Yeast Cell Stain (International Method)  [Released 2011]
B. Yeast Spore Stain  [Released 2011]
C. Triphenyltetrazolium Chloride for Identification of Respiratory-Deficient Cells  [Released 2011]

Yeast 4. Microscopic Yeast Cell Counting  [Released 2011, reviewed 2016]
VIEW SUMMARY | VIEW METHOD

Yeast 5. Yeast Solids 
VIEW SUMMARY | VIEW METHOD
A. Total by Dry Weight  [Released 2011]
B. Percent by Spin-Down  [Released 2011]

Yeast 6. Yeast Viability by Slide Culture  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 7. Yeast Sporulation  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 8. Killer Yeast Identification  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 9. Morphology of Giant Yeast Colonies (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 10. Differentiation of Ale and Lager Yeast 
VIEW SUMMARY | VIEW METHOD
A. X-α-GAL Medium  [Released 2011]
B. Growth at 37°C  [Released 2011]
C. Growth on Melibiose  [Released 2011]

Yeast 11. Flocculation 
VIEW SUMMARY | VIEW METHOD
A. Helm Assay  [Released 2011]
B. Absorbance Method  [Released 2011]

Yeast 12. Vitality By Fluorescence  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 13. Differentiation of Brewing Yeast Strains by PCR Fingerprinting  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 14. Miniature Fermentation Assay [Released 2012]
VIEW SUMMARY | VIEW METHOD

Yeast 15. Differentiation of Ale and Lager Yeast Strains by Rapid X-α-GAL Analysis [Released 2013]
VIEW SUMMARY | VIEW METHOD

Yeast 16. Phenolic Yeast Detection By Decarboxylation Of Ferulic Acid [Released 2016]
VIEW SUMMARY | VIEW METHOD

Microbiological Control

Microbiological Control 1. Aseptic Sampling 
VIEW SUMMARY | VIEW METHOD
A. Beer in Process  [Released 2011]
B. Packaged Beer  [Released 2011]
C. Process Water  [Released 2011]
D. Brewery Air  [Released 2011]
E. Process Equipment and Surfaces  [Released 2011]
F. Swab Surface Hygiene Using ATP Bioluminescence  [Released 2011]

Microbiological Control 2. Detection of Microorganisms 
VIEW SUMMARY | VIEW METHOD
A. Incubation  [Released 2011]
B. Plating  [Released 2011]
C. Membrane Filtration  [Released 2011]

Microbiological Control 3. Differential Staining  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Microbiological Control 4. General Culture Media 
VIEW SUMMARY | VIEW METHOD
A. Universal Beer Agar Medium  [Released 2011]
B. Brewers Tomato Juice Agar Medium  [Released 2011]

Microbiological Control 5. Differential Culture Media 
VIEW SUMMARY | VIEW METHOD
A. Cycloheximide Medium  [Released 2011]
B. Lee's Multi-Differential Agar  [Released 2011]
C. Raka-Ray Lactic Acid Bacteria Medium  [Released 2011]
D. Lysine Medium  [Release date 2011]
E. Lin's Wild Yeast Differential Medium  [Released 2011]
F. Barney-Miller Brewer Medium  [Released 2011]
G. DeMan Rogosa Sharpe Medium  [Released 2011]
H. MYGP + Copper Medium  [Released 2011]
I. CLEN Medium for Wild Yeast  [Released 2011]
J. Selective Medium for Megasphaera and Pectinatus (SMMP)  [Released 2011]
K. Nystatin in Selective Media  [Released 2011]
L. Hsu's Lactobacillus and Pediococcus Medium [Released 2009]

Microbiological Control 6. Water and Rinse Water Hygiene Using ATP [Released 2012]
VIEW SUMMARY | VIEW METHOD

Common Brewery-Related Microorganisms  [Released 2008]
VIEW SUMMARY | VIEW METHOD

Filter Aids Methods

Filter Aids 1. Sampling  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Filter Aids 2. pH of Water Suspension (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Filter Aids 3. Effect on Odor and Taste (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Filter Aids 4. Iron Pickup by Beer (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Packages and Packaging Materials Methods

Introduction
VIEW INTRODUCTION

Bottles

Bottles 1. Dimensions 1999 
VIEW SUMMARY | VIEW METHOD
A. Height  [Released 1999]
B. Outside Diameter  [Released 1999]
C. Out-of-Perpendicular  [Released 1999]
D. Identification Marks  [Released 1999]
E. Glass Distribution  [Released 1999]
F. Weight 1999 [Release date 1963]
G. Locking Ring "A" Diameter  [Released 1999]
H. Reinforcing Ring "B" Diameter  [Released 1999]
I.  Width of Locking Ring  [Released 1999]
J. Throat "I" Diameter  [Released 1999]
K. Finish  [Released 1999]

Bottles 2. Defects  [Released 1999]
VIEW SUMMARY | VIEW METHOD

Bottles 3. Color 
VIEW SUMMARY | VIEW METHOD
A. Amber Color  [Released 1999]
B. Redness Ratio  [Released 1999]

Bottles 4. Capacity 
VIEW SUMMARY | VIEW METHOD
A. Overflow  [Released 1999]
B. Fill Point  [Released 1999]

Bottles 5. Surface Protective Coatings 
VIEW SUMMARY | VIEW METHOD
A. Lubricity  [Released 1999]
B. Coating Quality  [Released 1999]
C. Rub Tests  [Released 1999]

Bottle Closures

Bottle Closures 1. Defects Glossary and Classification  [Released 1999]
VIEW SUMMARY | VIEW METHOD

Bottle Closures 2. Test Pressure 
VIEW SUMMARY | VIEW METHOD
A. Internal Pressure Test for Crowns  [Released 1995]
B. Internal Pressure Test for 28-mm Topside Pilferproof Closures  [Released 1995]
C. Internal Pressure Test for Plastic-Lined Convenience Two-Way Crown Applied to GPI 500 Series Finishes  [Released 1995]
D. Secure Seal Tester (SST) Internal Pressure Test  [Released 1995]

Bottle Closures 3. Gas Retention Capability of Crowns  [Released 1968]
VIEW SUMMARY | VIEW METHOD

Bottle Closures 4. Lithography Resistance to Pasteurization Conditions for Steel Crowns and Roll-On Pilferproof Aluminum Closures [Released 1999]
VIEW SUMMARY | VIEW METHOD

Bottle Closures 5. Removal Torque Procedure 
VIEW SUMMARY | VIEW METHOD
A. For Aluminum Closures  [Releasd 1993]
B. For Crowns  [Released 1993]

Bottle Closures 6. Crimp Determination Test -- Crowns  [Released 1994]
VIEW SUMMARY | VIEW METHOD

Cans

Cans 1. Defects Classification and Glossary for Seamless Two-Piece Cans 
VIEW SUMMARY | VIEW METHOD
A. Can and End Defects Classification  [Released 2004]
B. Can Defects  [Released 1999]
C. End Defects  [Released 1999]

Cans 2. Evaluation of Rusting Tendency of Beer Cans*  [Released 1970]

Cans 3. Dimensions 
VIEW SUMMARY | VIEW METHOD
A. Metal Gauge Thickness  [Released 2004]
B. Flange Width  [Released 2004]
C. Filled Can Countersink Depth  [Released 2004]

Cans 4. Ends 
VIEW SUMMARY | VIEW METHOD
A. Curl Opening  [Released 2004]
B. Seaming Chuck Fit  [Released 2004]
C. Ring-Pull-End Pop and Pull Test  [Released 2004]

Cans 5. Capacity 
VIEW SUMMARY | VIEW METHOD
A. Overflow  [Released 2004]
B. Headspace  [Released 2004]

Cans 6. Enamel Rater for Evaluating Metal Exposure  [Released 2004]
VIEW SUMMARY | VIEW METHOD

Cans 7. Beverage Can Terminology  [Released 2009]
VIEW SUMMARY | VIEW METHOD

Cans 8. Copper Sulfate Test  [Released 2009]
VIEW SUMMARY | VIEW METHOD

Fills

Fills 1. Total Contents of Bottles and Cans by Calculation from Measured Net Weight  [Released 1991]
VIEW SUMMARY | VIEW METHOD

Fills 2. Total Contents of Cans of Known Tare Weight  [Released 1991]
VIEW SUMMARY | VIEW METHOD

By-Products Methods

Introduction
VIEW INTRODUCTION

Barley

By-Products, Barley 1. Sampling  [Released 1999]
VIEW SUMMARY | VIEW METHOD

By-Products, Barley 2. Physical Tests  [Released 1999]
VIEW SUMMARY | VIEW METHOD

By-Products, Barley 3. Moisture  [Released 1999]
VIEW SUMMARY | VIEW METHOD

By-Products, Barley 4. Protein  [Released 1999]
VIEW SUMMARY | VIEW METHOD

Malt

By-Products, Malt 1. Sampling  [Released 1999]
VIEW SUMMARY | VIEW METHOD

By-Products, Malt 2. Physical Tests  [Released 1999]
VIEW SUMMARY | VIEW METHOD

By-Products, Malt 3. Moisture  [Released 1999]
VIEW SUMMARY | VIEW METHOD

By-Products, Malt 4. Protein  [Released 1999]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis Methods

Introduction
VIEW INTRODUCTION

Beer Flavor Database  [Released 2011]
VIEW SUMMARY  |   VIEW SPREADSHEET (Excel)  |   VIEW SPREADSHEET (PDF)

Sensory Analysis 1. Terms and Definitions (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 2. Test Room, Equipment, Conduct of Test (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 3. Choice of Method (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 4. Selection and Training of Assessors (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD
A. Selection and Training of Assessors (International Method) [Released 2011]
B. Training Method: Brand Description Generation and Evaluation [coming soon]
C. Training Method: QA/Production-Focused [coming soon]
D. Training Method: Descriptive Analysis [coming soon]

Sensory Analysis 5. Author Guidelines for Reporting (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 6. Paired Comparison Test (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 7. Triangular Test (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 8. Duo-Trio Test (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 9. Threshold of Added Substances -- Ascending Method of Limits Test (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 10. Descriptive Analysis (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 11. Ranking Test (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 12. Flavor Terminology and Reference Standards (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 13. Difference-from-Control (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 14. Hot Steep Malt Sensory Evaluation Method  [Released 2017]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 15. Hop Tea Sensory Method  [Released 2017]
VIEW SUMMARY | VIEW METHOD

Statistical Analysis Methods

Introduction
VIEW INTRODUCTION

Statistical Analysis 1. Linear Calibration  [Released 1983]
VIEW SUMMARY | VIEW METHOD

Statistical Analysis 2. Limits of Detection and Determination  [Released 1983]
VIEW SUMMARY | VIEW METHOD

Statistical Analysis 3. Ruggedness Testing  [Released 1983]
VIEW SUMMARY | VIEW METHOD

Statistical Analysis 4. Youden Unit Block Collaborative Testing Procedure  [Released 1983]
VIEW SUMMARY | VIEW METHOD

Statistical Analysis 5. Comparison of Test Methods  [Released 1986]
VIEW SUMMARY | VIEW METHOD

Statistical Analysis 6. Comparison of Microbiological Data  [Released 1987]
VIEW SUMMARY | VIEW METHOD

Appendix I - t-Distribution (Two-tailed Tests)
VIEW SUMMARY | VIEW APPENDIX

Appendix II - F-Distribution, = 0.05
VIEW SUMMARY | VIEW APPENDIX

Appendix III - F-Distribution, = 0.025
VIEW SUMMARY | VIEW APPENDIX

Appendix IV - F-Distribution, = 0.01
VIEW SUMMARY | VIEW APPENDIX

Appendix V - F-Distribution, = 0.005
VIEW SUMMARY | VIEW APPENDIX

Appendix VI - F-Distribution, = 0.001
VIEW SUMMARY | VIEW APPENDIX

Appendix VII - Cumulative Distribution of Chi-Square
VIEW SUMMARY | VIEW APPENDIX

Appendix VIII - Definition of Statistical Terms  [Released 1987]
VIEW SUMMARY | VIEW APPENDIX

Appendix Procedure

I Instrument Standardization 
VIEW APPENDIX
A. Calibration of Ultraviolet-Visible Spectrophotometer (International Method)  [Released 1972]

II Nomenclature of Hop Resin Fractions and Components  [Released 1969]
VIEW APPENDIX

III Water Analysis  [Released 1971]
VIEW APPENDIX

Tables

Tables for Extract Determinations in Malt and Cereals
VIEW SUMMARY | VIEW TABLES

Tables Related to Determinations on Wort, Beer, and Brewing Sugars and Syrups
VIEW SUMMARY | VIEW TABLES

Table 1. Extract

Table 2. Alcohol

Table 3. Original Extract (correction factors)

Table 4. Reducing Substances (Munsun-Walker)

Table 5. Reducing Substances (Lane-Eynon)

Table 6. Baume Degrees

Identification Guides

Beer Inclusions: Common Causes of Elevated Turbidity
VIEW SUMMARY | VIEW GUIDE

Common Brewery-Related Microorganisms
VIEW SUMMARY | VIEW GUIDE

Flavor Standard Spiking Calculator
VIEW SUMMARY | VIEW CALCULATOR

Beer Flavor Database  [Released 2011]
VIEW SUMMARY  |   VIEW SPREADSHEET (Excel)  |   VIEW SPREADSHEET (PDF)

Hop Flavor Database
VIEW SUMMARY  |   VIEW SPREADSHEET (Excel)   |   VIEW SPREADSHEET (PDF)

Recommended Reading
VIEW SUMMARY | VIEW DOCUMENT

* Archived. Reports may be obtained in the "Archived Methods" section of this site.