ASBC Methods of Analysis - Wort-19: Fermentable Carbohydrates by Cation Exchange HPLC
ASBC Methods of Analysis
Fermentable Carbohydrates by Cation Exchange HPLC
Diluted worts are injected into a high-performance liquid chromatography system. A cation exchange column with water as mobile phase is used to separate the sugars, which are then measured by a refractive index detector. Sugars having a degree of polymerization of 1-3 are quantitatively determined by comparison with aqueous standards containing fructose, glucose, maltose, and maltotriose.