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ASBC Methods of Analysis - Wort-19: Fermentable Carbohydrates by Cation Exchange HPLC

ASBC Methods of Analysis

Wort

Wort-19
Fermentable Carbohydrates by Cation Exchange HPLC

VIEW METHOD

Summary
Diluted worts are injected into a high-performance liquid chromatography system. A cation exchange column with water as mobile phase is used to separate the sugars, which are then measured by a refractive index detector. Sugars having a degree of polymerization of 1-3 are quantitatively determined by comparison with aqueous standards containing fructose, glucose, maltose, and maltotriose.