ASBC Methods of Analysis - Wort-18: ß-Glucan in Congress Wort
ASBC Methods of Analysis
ß-Glucan in Congress Wort
This method measures the ß-glucan content of congress wort by two techniques. The first exploits the chemistry of the fluorochrome Calcofluor to bind to 1,3- and 1,4-ß-D-glucan. Flow injection analysis is utilized to quantify ß-glucan in congress wort. Segmented flow analysis is used in the second technique. The principle of the methodology involves diluting the wort sample with distilled water and mixing with a Calcofluor reagent, which results in the formation of a fluorescent adduct, cellufluor-ß-glucan (1). The fluorescence intensity of the complex is measured. The fluorescence peak height of the complex is proportional to the concentration of ß-glucan in the wort sample.