ASBC Methods of Analysis - Pouring Agar Plates Video

ASBC Methods of Analysis

Pouring Agar Plates Video


This video introduces the concept of preparing agar plates by pouring culture medium into a sterile petri dish and allowing it to solidify in a sterile environment. This technique is important for many basic microbiological analyses within the quality-control laboratory, such as for the detection of microorganisms in beer or process samples or for the preparation and short-term storage of yeast cultures.

Notes: In this video, a Bunsen burner is used to produce a sterile environment. However, a sterile laminar-flow cabinet or equivalent can also be used. If neither of these pieces of equipment is available, it is important to ensure that the work surface and the poured plates are microbiologically clean. This can achieved by wiping surfaces with 70% ethanol and immediately replacing the lid of the petri dish after pouring. In this instance, to avoid condensation buildup, plates should be places in an incubator for 24 h to dry sufficiently prior to use. This method may also serve as a rough check to ensure that poured plates are not contaminated with fast-growing organisms. Irrespective of the mode of preparation, prepared plates should be stored at 4°C for no longer than 2 weeks prior to use.