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ASBC Methods of Analysis - Microbiological Control-4: General Culture Media

ASBC Methods of Analysis

Microbiology

Microbiological Control-4
General Culture Media

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Summary
This method provides instructions for preparing two media (universal beer agar and brewers’ tomato juice medium) that can be used for general microbiological control work in the brewery. Most culture yeast, nonculture yeast, and Gram-negative rods commonly found in breweries grow well on these media under standard conditions. Lactic acid organisms commonly found in breweries, such as Lactobacilli and Pediococci, grow well on these media under anaerobic conditions. When supplemented with cycloheximide, these media differentially suppress the growth of brewing yeast. A safer alternative to cycloheximide is nystatin.