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ASBC Methods of Analysis - Malt-16: Lipoxygenase Enzymatic Activity In Malted Barley By Absorbance Method

ASBC Methods of Analysis

Malt

Malt-16
Lipoxygenase Enzymatic Activity In Malted Barley By Absorbance Method

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Summary
Lipoxygenase (LOX) is first extracted from malted barley with an acetate buffer. The extracted LOX is combined with an aliquot of linoleic acid. Spectroscopy is utilized to measure the enzymatic activity of LOX, which can be seen as an increase of absorbance at 234 nm.