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ASBC Methods of Analysis - Barley-10: Barley Pregermination by Fluorescein Dibutyrate

ASBC Methods of Analysis

Barley

Barley-10
Barley Pregermination by Fluorescein Dibutyrate

VIEW METHOD

Summary
This method is designed to detect and measure the level of pregerminated grains in harvested barley. It can be used to check barley for pregermination at receiving stations before it is committed to storage. This method is based on the principle that germination is characterized by the release of enzymes that can be visually identified by staining with a lipase-sensitive fluorochrome. The enzyme activity appears as bright yellow fluorescence when viewed under ultraviolet light.