Prefaces to earlier editions
PREFACE TO THE 2009 EDITION
The 2009 ASBC Methods of Analysis (MOA) is a direct result of the efforts of the society to fulfill its vision to create and disseminate scientific brewing knowledge, continuously improve and expand methods of measurement, proactively identify and rapidly respond to industry technical concerns, and develop world-class scientists for the brewing industry.
The 2009 MOA has been upgraded with numerous additions and enhancements and, as with previous editions, is an important resource for brewing scientists around the globe. Of the three new collaboratively tested and approved analytical methods added to the 2009 MOA, two address the industry need for measurements to predict flavor stability. Beer-46 measures oxidative resistance and free radical production in beer using a forced oxidation assay, and Wort-21 quantifies the effects of thermal stress on wort and beer. Both methods provide the brewer with tools to monitor and adjust processes to improve flavor stability.
The new Yeast-13 provides a technique for PCR fingerprinting to differentiate and identify different brewing yeast strains. And finally, a section has been added to Beer-13 that enables the determination of dissolved carbon dioxide in beer by an instrumental method using the principle of volume expansion.
An extensive review of the entire MOA hop section resulted in revisions for the 2009 edition, featuring updated methods to reflect best practices and an enhanced hop glossary with additional information. Several method-specific calculators were added to the 2009 MOA to continue to add value for the user and assist in implementing the methods with ease.
I express my gratitude for the countless hours and hard work that many individuals representing many organizations from around the world put into this effort—the 2009 edition of the ASBC MOA. The MOA would not be possible without the input from these volunteers, beginning with the collaborators on each of the subcommittees. It would be a daunting task to list all of the contributors by name, but I would like to mention the efforts of the Technical Committee led by Dana Sedin and the members of the Publication Committee and editorial team. A special thank you is also extended to ASBC staff for their continuous support and guidance.
Publications Committee Chair
PREFACE TO THE 2008 EDITION
The ASBC Methods of Analysis has undergone significant changes since the conversion to a CD-ROM format in 2004. With annual updates since then, MOA has become more essential than ever as a brewing resource with 225 subcommittee-evaluated methods and many other enhancements, all intended as value-added tools for brewing scientists around the world. In the 2008 update of MOA, the user will find more than 70 electronic Calculators linked to methods, an expansive Glossary of Brewing Terms, a statistics tutorial entitled Statistics Using Youden Unit Block Design, in addition to the following new enhancements: an image library of Common Brewery-Related Microorganisms, a Beer Inclusions Guide, a Reference Section of Brewing-Related Textbooks, and numerous other features.
As always, revisions of Methods of Analysis has been a collaborative effort that would not be possible without the continued dedication of several individuals comprising the Publications Committee, the Technical Committee, and staff at Scientific Societies. Special thanks to Mike Joyce, Greg Grahek, and Steve Kronmiller for their support, hard work, creativity, and commitment to Methods of Analysis and ASBC.
Cindy-Lou Dull & Susan Kay
PREFACE TO THE 2006 EDITION
The 2006 edition continues the innovation allowed by a digital medium. This CD-ROM contains the first recorded ASBC tutorial designed to enhance your ability to better utilize the methods. This tutorial, “Statistics Using Youden Unit Block Design,” is written and narrated by James Munroe and is the first in a series of enhancements that will help brewing scientists attain a new level of success in understanding and utilizing the Methods of Analysis.
A new Glossary covering terms in the Beer section arrives with this 2006 Edition, as well. This glossary will be expanded to include other sections in forthcoming updates in 2007 and 2008. We have also added a sequence of color photographs to demonstrate steps in the Total Sulfur Dioxide method (Beer-21). Watch for more helpful demonstration photograph sequences in the forthcoming updates.
We have added eight new built-in calculators, and the 2006 Methods of Analysis now offers more than 65 in all. We continue to add links, and the built-in search functionality will help you find everything you need, including five new or revised methods—one in Barley, one in Hops, two in Beer, and one in Microbiology.
I thank all the contributors to this new edition, including the Technical Committee and its subcommittees and the Editorial Committee. Together, we continue to improve the ASBC Methods of Analysis to ensure accuracy of methods and maintain consistent high quality in brewing science methodology.
Publications Committee Chair
PREFACE TO THE NINTH EDITION
The general format of the methods in the ninth edition is similar to that in preceding editions, with one monumental change—moving the methods to an electronic computer compact disc (CD) medium.
The ninth edition does encompass a number of changes. Moving into the electronic age, the ninth edition has made the transition from the loose-leaf paper format into an easy-to-use computer CD, with many enhanced features. All methods are easy to locate from the table of contents, with direct links to each method. Enhanced searchable features provide easy retrieval of all the methods. Calculators are linked to many of the methods so that the user can easily perform calculations. Equations have been changed from the linear format to the more-easy-to-understand stackable format. The ninth edition also includes the methods that were in the two supplements to the eighth edition and the 21 new methods (including an expanded packaging methods section) that were approved by the Technical Committee and its subcommittees since the release of the last supplement in 1999.
As in the past, revisions of Methods of Analysis has been a collaborative project involving the Executive Committee of the Society, the Technical Committee and its subcommittees, and the Editorial Committee. The ninth edition is a compilation of the tremendous work and vision of everyone who has had a position of responsibility in the ASBC organization since the release of the eighth edition. Although it is impossible to individually thank all the members who have assisted in this work, I would like to thank Margaret Morrison and Dick Pyler for their countless hours in reviewing the methods; Cindy-Lou Dull, chairperson of the Technical Committee and her technical chairs for the tremendous amount of time reviewing the methods for technical content and safety provisions, and the past ASBC board for their guidance, support, and hours devoted to reviewing methods. A special thanks to the entire staff at the Society’s offices, for their tremendous patience, advice, and assistance.
Michael J. Joyce
Publications Committee Chair
PREFACE TO THE EIGHTH EDITION
The general format of the methods in the eighth edition is similar to that in preceding editions. In addition, the principles involved in the choice of methods to be included remain unchanged. The reader is referred to the preface of the seventh edition for an excellent discussion.
The eighth edition does encompass a number of changes. While the loose-leaf format has been retained so as to facilitate future additions and changes, page size has been increased, allowing two columns of text per page. This has decreased the overall number of pages. In addition, a number of obsolete methods have been removed from the text. Copies of these “archived” methods may be obtained, if wanted, by writing the Society offices in St. Paul. The methods so archived are noted in the tables of contents of the different chapters.
In the interest of laboratory safety, the use of mercury and mercury compounds has been eliminated in nitrogen (protein) determinations, and benzene has been deleted as a recommended solvent in hops analyses.
Twenty-one new methods resulting from subcommittee evaluation since publication of the 1987 Supplement have been added in this edition. Also, by agreement with The Beer Institute, 34 methods for evaluating packages and packaging materials formerly promulgated by the United States Brewers Association have been incorporated. These methods are considered provisional pending collaborative study by the Packaging Division and rewriting in the style normally employed in Methods of Analysis.
Tables Related to Determinations on Wort, Beer, and Brewing Sugars and Syrups and Tables for Extract Determination in Malt and Cereals have been reprinted and are now incorporated as new chapters of Methods of Analysis instead of being issued separately as booklets.
Following discussions with the AOAC and EBC, the Society has retained the use of specific gravity rather than density in many of the methods described in this book. Although density would be the correct unit in most of these methods, the differences are extremely small and this, together with other considerations, led to agreement not to change the existing methods.
The calculation of carbon dioxide content of beer by volume in Beer-13 has been corrected by incorporating the commonly accepted standard molar volume of CO2 (22.272 L).
As in the past, revision of Methods of Analysis has been a collaborative project involving the Executive Committee of the Society, the Technical Committee and its subcommittees, and the Editorial Committee. Although it is impossible to individually thank all the members who have assisted in this work, I particularly wish to express appreciation to James Munroe and Robert McCaig, who, as chairmen of the past two Technical Committees, have provided invaluable assistance. Thanks are also due to staff at the Society’s offices, for helpful advice and untiring patience.
John A. Thorn
PREFACE TO THE SEVENTH EDITION
The general format of presentation of the methods in this seventh edition is similar to that in the preceding edition. Where appropriate, an introduction to the method, or methods, has been used. This is followed by a listing of reagents and apparatus required. Where applicable, a section on calibration is used, followed by the method, calculation, and application of the calculation in the form of a specific example. The numbers in such examples could occur in typical samples but are not intended to represent actual or expected results of an analysis. Notes have been used for additional clarity. References are given which may, or may not, be cited in the text of the method. Customarily, these report the action of ASBC subcommittees in modifying existing methods or in proposing new or alternate procedures. Additional references providing background for or substantiating findings of the subcommittee have been used where appropriate.
All of the text of the previous sixth edition has been carefully reviewed and revised in the interest of accuracy, clarity, and uniformity in the use of nomenclature, numbers, and symbols. Advancing technology has rendered a number of the methods published in previous editions somewhat obsolete. However, if such methods are judged to be in use at all or are valuable for reference, they are reproduced in an updated form. Many new methods have resulted from ASBC subcommittee work and new chapters have also been added.
The methods included in this seventh edition are those adopted by the Society through May, 1976. Undoubtedly, additional methods, deletions, and modifications will result in the succeeding years before a subsequent edition is justified. Accordingly, the Methods of Analysis has been structured such that deletions, modifications, and insertions are facilitated. Continuous pagination throughout the book has been eliminated, and detailed Tables of Contents have been restricted to each individual chapter. For example, in the 1958 edition 20 sections were listed under beer analysis; these now number 33 and others are contemplated to meet the growing requirements of the Industry. Chapters on Filter Aids and on Packages and Packaging Materials have been added, and the chapter on Yeast has been expanded to include the advancing technology of microbiology in general. Procedures having general application to many methods are given in an Appendix, as are detailed references of interest from authoritative sources. The reader will note a significant deletion from the previous edition—a “Suggested Form of Report” is not included with each chapter. Serious consideration indicated that no common style of report would satisfy the needs of individual companies and organizations.
As rapidly as practical the methods are being converted to metrication—in its simplest form the classical centimeter-gram-second (CGS) system. Liter (1.) and milliliter (ml) are customary volume designations, and temperature is given in degrees Celsius unless otherwise indicated. In addition, a supplemental International System of Units (SI) has been introduced where applicable, since these are recognized in all languages.
Some of the methods are titled “International Method,” indicating an additional approach to a “common language” in the Industry. The designation International Method, or “IM,” assigned to analytical methods of the ASBC, indicates that agreement has been reached between the ASBC and the European Brewery Convention (EBC) that the methods so designated are sufficiently close in methodology, etc., as to warrant International Method status. For analytical methods of ASBC and EBC to qualify as International, essentially the methods and results should be the same, allowing for small differences in detailed procedure. If the methods produce the same results and the methodology is not in conflict, the wording need not be identical. For example, ASBC methods may be printed in more detail than EBC methods. Brand names may not necessarily be stipulated by either ASBC or EBC but the methods should include the necessary characteristics of the items involved. Other guidelines and information pertaining to International Methods may be found in Proc. Amer. Soc. Brew. Chem. 33(3):93(1975), and in subsequent reports of the ASBC Subcommittee on International Methods.
The methods outlined are designed to permit a high degree of precision. Accordingly, good laboratory practice is assumed in such matters as accuracy of apparatus, purity of reagents, and precision in their use. Familiarity with the principles of analytical chemistry and, where required, in such other areas as microbiology are assumed. These are supplemented by the specifics given for each individual method. It is understood that laboratories may devise modifications of individual methods in the interest of speed or simplification. Any such modification will require extensive study to ensure that results comparable to the standard reference method are achieved, both within and between laboratories.
Terminology and abbreviations throughout the methods are, with minor exceptions, consistent with those of the American Chemical Society, and are those recommended for use in the Journal of the ASBC. Absorbance (A) has replaced optical density (O.D.) and nanometers (nm) has replaced millimicrons (mμ). Parts per million (ppm) and parts per billion (ppb) are now expressed in units giving greater clarity and flexibility. For example, w/v or w/w relations may be expressed as mg/l. or mg/kg instead of ppm, and μg/l. or μm/kg instead of ppb; other variants, such as μg/ml or μg/g for “ppm,” are obvious. For clarity throughout the methods, any abbreviation not well understood is spelled out before use and unusual abbreviations are avoided.
A set of tabbed dividers covering the now-current Chapters is provided. These are not arranged alphabetically but in such a fashion as to approximate entrance into the brewing process from barley and malt through packages and packaging materials. It is suggested that the book be maintained in this order.
The two sets of tables published by the Society, “Tables Related to Determinations on Wort, Beer, and Brewing Sugars and Syrups,” and “Tables for Extract Determination in Malt and Cereals,” have been carefully reviewed and errors corrected where found. The user will note that minor errors are inherent to cumulative rounding-off; essentially, these are insignificant and well within the limits of experimental error. Both sets of tables have been reprinted and are included with the seventh edition.
Revision of the Methods of Analysis has been a collaborative project involving the Executive Committee of the Society, the Technical Committee and its subcommittees, and the Editorial Committee. It would be impossible to list all those members of the Society who have given freely of their time to assist in the revision. However, special appreciation is due Dwight B. West whose untiring efforts not only led to publication of the previous sixth edition but whose guidance as Editorial Committee Chairman initiated the revision of this seventh edition.